The genus Silphium, better known as the “Rosin-weeds”, belongs to the sunflower family Asteraceae. Silphium is native to North America and has species that stretch across the eastern United States, parts of Canada, and the midwest. Missouri is home to 4 native species of Silphium. Silphium has been identified as a potential source of biofuels. Silphium was last studied in 2000 and therefore requires reevaluation with modern DNA analyses methods. We utilized the bioinformatics programs MrBayes on XSEDE and BioEdit to help us understand whether signatures of evolution would vary among different species, taxonomical sections, and subsections within the group based on the data we derived from nucleic and chloroplast markers. Combining data from a variety of loci has allowed us to develop a better understanding of the relationships present between members of the Silphium genus.